Application
Figure A. FITC conjugated mouse anti human CD45RA and RPE-Alexa647® conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated mouse anti human CD45RA and RPE-Cy5 conjugated mouse anti human CD45RO . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes.| Product Name | CD45RO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [UCHL1] to CD45ROThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | UCHL1 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-PIP |
| Flow Cytometry: 1/25 - 1/50 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanChimpanzeeMarmoset |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD45 LY5 T200 L-CA PTPRC LCA CD45R B220 GP180 |
|---|---|
| Target | CD45RO |
| Tissue Specificity | This antibody recognizes the low molecular weight isoform (180kD) of CD45. |
Application
Figure A. FITC conjugated mouse anti human CD45RA and RPE-Alexa647® conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated mouse anti human CD45RA and RPE-Cy5 conjugated mouse anti human CD45RO . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes.| Application Notes | Flow Cytometry: 1/25 - 1/50 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
