Application
Western Blot analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5) . Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; )| Product Name | CD59 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 43 / 5] to CD59CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. |
| Immunogen | Thymocytes and T lymphocytes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MEM-43/5 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MIN2 MIN3 PROTECTIN MSK21 CD59 MIC11 966 107271 MIN1 P13987 MGC2354 16.3A5 1F5 EJ16 EJ30 EL32 FLJ38134 FLJ92039 G344 HRF-20 HRF20 MAC-IP MACIF MEM43 MIRL p18-20 HRF 20 MAC IP MACIP |
|---|---|
| Target | CD59 |
| Tissue Specificity | CD59 antigen (human). MEM-43/5 reacts with well defined epitope (around L33) and does not compete with MEM-43 and many other CD59 antibodies |
| Cellular Localization | Cell Membrane |
Application
Western Blot analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5) . Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; )| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
