Application
Surface staining of human peripheral blood cells with anti-human CD6: FITC .| Product Name | CD6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-98] to CD6CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells. |
| Immunogen | CD6 antigen purified by immunoaffinity chromatography from HBP ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-98 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB (non-reducing conditions): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 186720 CD6 P30203 923 TP120 FLJ44171 |
|---|---|
| Target | CD6 |
| Tissue Specificity | This antibody reacts with CD6, a 100-130 kD single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells. |
Application
Surface staining of human peripheral blood cells with anti-human CD6: FITC .| Application Notes | For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB (non-reducing conditions): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
