Application
Western blot analysis of L-Selectin expression in Jurkat cell lysate.| Product Name | CD62L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD62LSelectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naive T cells and on most monocytes, neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells, monocytes, and neutrophils. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes L-Selectin in human sample, and in a lesser extent to mouse, and rat samples.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SELL Lymphocyte adhesion molecule1 Lymphocyte adhesion molecule 1 L Selectin LSelectin |
|---|---|
| Target | CD62L |
| Tissue Specificity | This antibody is specific for L-Selectin/CD62L. |
Application
Western blot analysis of L-Selectin expression in Jurkat cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes L-Selectin in human sample, and in a lesser extent to mouse, and rat samples.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
