Application
Immunohistochemistry (IHC) analyzes of CD63 pAb in paraffin-embedded human lung carcinoma tissue.Application
Western blot (WB) analysis of CD63 pAb at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysateWB
(C) Western blot results of tetraspanins CD9 and CD63 expression in exosomes.WB
E The relative expression levels of CD9, CD63, and TSG101 were analyzed by a Simple Wes System. The quantification of the intensity of exosome markers were shown in the right panels.| Product Name | CD63 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD63The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| Immunogen | Synthetic peptide, corresponding to amino acids 111-160 of Human CD63. |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | LAMP3 ME491 CD63 molecule OMA81H TSPAN30 MLA1 |
|---|---|
| Target | CD63 |
| Tissue Specificity | CD63 (K156) pAb detects endogenous levels of CD63 protein. |
Application
Immunohistochemistry (IHC) analyzes of CD63 pAb in paraffin-embedded human lung carcinoma tissue.
Application
Western blot (WB) analysis of CD63 pAb at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate
WB
(C) Western blot results of tetraspanins CD9 and CD63 expression in exosomes.
WB
E The relative expression levels of CD9, CD63, and TSG101 were analyzed by a Simple Wes System. The quantification of the intensity of exosome markers were shown in the right panels.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | supplied in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
