Subcellular fraction of human alveolar macrophages.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
KP1
Host Species
Mouse
Tested Applications
IHC-FIHC-P
This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. High temperature antigen unmasking with 10mM citrate buffer, pH6.0 prior to staining.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
153634 968 P34810 CD68 SCARD1
Target
CD68
Tissue Specificity
This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Cellular Localization
Cell membrane
Light Chain Type
kappa
Application
Application
IHC-P analysis of human tonsil using CD68 antibody [KP1].
Positive Control
Tonsil
Application Notes
This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. High temperature antigen unmasking with 10mM citrate buffer, pH6.0 prior to staining.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.