Mouse monoclonal [HM47 / A9] to CD79a predilutedCD79a is a disulphide-linked heterodimer, consisting of CD79a / mb-1 and CD79b / B29 and is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell antigen receptor. CD79a first appears at pre B cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas and in some myelomas.
Immunogen
Synthetic polypeptide consisting of the amino acid sequence GTYQDVGSLNIADVQ.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HM47/A9
Host Species
Mouse
Tested Applications
IHC-P
IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Synonyms
MB-1 CD79A MB1 112205 973 IGA P11912 MB 1
Target
CD79a
Cellular Localization
Cell Membrane
Light Chain Type
kappa
Application
Application
IHC-P analysis of human tonsil using CD79a antibody [HM47/A9].
Positive Control
Tonsil
Application Notes
IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.