Application
Human Tonsil stained with anti-CD8 antibodyApplication
Human Endometrium stained with anti-CD8 antibodyApplication
Human Cervix stained with anti-CD8 antibodyApplication
Human Colon stained with anti-CD8 antibodyApplication
Human B Cell Lymphoma stained with anti-CD8 antibodyApplication
Human HK Lymphoma stained with anti-CD8 antibodyApplication
Human Spleen stained with anti-CD8 antibodyApplication
Human Reactive Lymph Node stained with anti-CD8 antibodyApplication
Human Thymus stained with anti-CD8 antibodyApplication
Human Colon Adenocarcinoma stained with anti-CD8 antibodyApplication
Human Thyroid stained with anti-CD8 antibodyApplication
Human Esophagus stained with anti-CD8 antibodyApplication
Human Prostate stained with anti-CD8 antibodyApplication
Human Fallopian Tube stained with anti-CD8 antibody| Product Name | CD8 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Rabbit Antibody to Human CD8The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | SP16 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 186910 926 LYT3 MAL Leu2 CD8B1 CD8A P10966 MGC119115 p32 Ly3 CD8 CD8B 925 186730 P01732 |
|---|---|
| Target | CD8 |
| Tissue Specificity | This antibody recognizes CD8. CD8 molecule consists of two chains, termed alpha and Beta chain, which are expressed as a disulphide-linked alpha/Beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/Beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not Beta chain).This antibody crossreacts with Human. |
| Cellular Localization | Cell membrane |
Application
Human Tonsil stained with anti-CD8 antibody
Application
Human Endometrium stained with anti-CD8 antibody
Application
Human Cervix stained with anti-CD8 antibody
Application
Human Colon stained with anti-CD8 antibody
Application
Human B Cell Lymphoma stained with anti-CD8 antibody
Application
Human HK Lymphoma stained with anti-CD8 antibody
Application
Human Spleen stained with anti-CD8 antibody
Application
Human Reactive Lymph Node stained with anti-CD8 antibody
Application
Human Thymus stained with anti-CD8 antibody
Application
Human Colon Adenocarcinoma stained with anti-CD8 antibody
Application
Human Thyroid stained with anti-CD8 antibody
Application
Human Esophagus stained with anti-CD8 antibody
Application
Human Prostate stained with anti-CD8 antibody
Application
Human Fallopian Tube stained with anti-CD8 antibody| Positive Control | Tonsil |
|---|---|
| Application Notes | We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
