Application
Flow cytometric analysis of jurkat cells using CD8B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-CD8B1 Pab in K562 cell line lysates (35ug/lane).| Product Name | CD8B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-cell surface glycoprotein CD8 beta chain, CD8b, CD8B, CD8B1 |
| Product description | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.1) Nakayama,K., J. Immunol. 148 (6), 1919-1927 (1992) |
| Immunogen | This CD8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the N-terminal region of human CD8B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD8B |
|---|---|
| Alternative Names | T-cell surface glycoprotein CD8 beta chain CD8b CD8B CD8B1 |
| Molecular Weight(MW) | 24 kDa |
Application
Flow cytometric analysis of jurkat cells using CD8B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-CD8B1 Pab in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
