Application
WB analysis of Jurkat cells using CD8B antibody at 0.2-1μg/ml. Lane A : marker Lane B : Jurkat cells| Product Name | CD8 beta antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD8 betaThe CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human CD8B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Dog: 93%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Rat: 86% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD8B1 LYT3 CD8B LEU2 P37 LY3 |
|---|---|
| Target | CD8B |
| Molecular Weight(MW) | 24 KDa (note) |
Application
WB analysis of Jurkat cells using CD8B antibody at 0.2-1μg/ml. Lane A : marker Lane B : Jurkat cells| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
