Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody.Application
HEK293T cells transfected with either RC206540 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD80 antibody, and then analyzed by flow cytometry.Application
Anti-CD80 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CD80Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.| Product Name | CD80 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E9] to CD80Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| Immunogen | Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100,: |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD28LG CD28LG1 CD80 LAB7 |
|---|---|
| Target | CD80 |
| Tissue Specificity | is specific for human CD80 (NP_005182). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody.
Application
HEK293T cells transfected with either RC206540 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD80 antibody, and then analyzed by flow cytometry.
Application
Anti-CD80 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CD80
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.| Application Notes | ICC/IF 1:100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
