Application
Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80: APC . Total viable cells were used for analysis.| Product Name | CD80 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 233] to CD80CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. |
| Immunogen | Fusion protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-233 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | CD28LG CD80 941 112203 CD28LG1 P33681 LAB7 |
|---|---|
| Target | CD80 |
| Tissue Specificity | CD80 (B7-1) |
| Cellular Localization | Cell Membrane |
Application
Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80: APC . Total viable cells were used for analysis.| Application Notes | For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
