Application
Western blot analysis of extracts of various cell lines, using CD86 antibody.Application
Immunofluorescence analysis of MCF-7 cell using CD86 antibody. Blue: DAPI for nuclear staining.| Product Name | CD86 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD86This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Immunogen | Recombinant protein of human CD86 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:100, IF 1:50 - 1:100, IP 1:50 - 1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B7.2 LAB72 CD86 molecule B70 CD28LG2 B7-2 |
|---|---|
| Target | CD86 |
| Molecular Weight(MW) | 38 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using CD86 antibody.
Application
Immunofluorescence analysis of MCF-7 cell using CD86 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:100, IF 1:50 - 1:100, IP 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
