Application
Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9: FITC . Total viable cells were used for analysis.| Product Name | CD9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 61] to CD9CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). |
| Immunogen | Tissue / cell preparation (Human). (Pre-B line Nalm-6). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-61 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PWB |
| FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. The antibody induces FcgR-dependent platelet activation (aggregation). IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRP-1 MIC3 CD9 BTCC1 143030 BTCC-1 BA2 DRAP27 5H9 TSPAN29 GIG2 DRAP-27 928 P21926 MRP1 P24 MRP 1 BTCC 1 DRAP 27 |
|---|---|
| Target | CD9 |
| Tissue Specificity | This antibody reacts with human CD9 antigen. |
Application
Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9: FITC . Total viable cells were used for analysis.| Application Notes | FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. The antibody induces FcgR-dependent platelet activation (aggregation). IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
