Application
Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9: FITC . Total viable cells were used for analysis.| Product Name | CD9 antibody (Azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MEM-61] to CD9CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. |
| Immunogen | Pre-B cell line NALM-6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-61 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PWBFunctional Assay |
| For FACS: Use at an assay dependent diluiton. For FuncS: Use at an assay dependent dilution. Induces FcgR-dependent platelet activation (aggregation). For WB: Use at a concentration of 2-4 μg/ml under non-reducing conditions. Optimal dilutions/concentrations should be determined by the researcher: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MIC3 BA2 DRAP27 5H9 928 MRP-1 CD9 BTCC1 143030 BTCC-1 TSPAN29 GIG2 DRAP-27 P21926 MRP1 P24 MRP 1 BTCC 1 DRAP 27 |
|---|---|
| Target | CD9 |
| Tissue Specificity | Recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a protein of 24 kDa belonging to tetraspans family (TM4SF). |
Application
Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9: FITC . Total viable cells were used for analysis.| Application Notes | For FACS: Use at an assay dependent diluiton. For FuncS: Use at an assay dependent dilution. Induces FcgR-dependent platelet activation (aggregation). For WB: Use at a concentration of 2-4 μg/ml under non-reducing conditions. Optimal dilutions/concentrations should be determined by the researcher: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 |
