Application
Sample (30 ug of whole cell lysate) A: THP-1 15% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed H1299, using CD99 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CD99 antibody at 1:500 dilution.| Product Name | CD99 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD99 (CD99 molecule)The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CD99. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBA71 antibody MIC2 antibody MIC2X antibody MIC2Y antibody MSK5X antibody CD99 antibody T-cell surface glycoprotein E2 antibody MIC2 (monoclonal antibody 12E7) antibody CD99 antigen antibody E2 antigen antibody antigen identified by monoclonal an |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) (7) |
| Cellular Localization | Membrane |
Application
Sample (30 ug of whole cell lysate) A: THP-1 15% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed H1299, using CD99 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CD99 antibody at 1:500 dilution.| Positive Control | K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
