Mouse monoclonal [HO36-1.1] to CD99 Ewing SarcomaCD99 is a cell surface antigen expressed in 95% of Ewing's sarcomas (ES). The antigen is also found in some primative neuroectodermal tumors (PNET) and peripheral neuroepitheliomas (PN). Other small round cell tumors (SRCT) that are commonly included in the differential diagnosis of ES, such as neuroblastomas, lymphomas, and rhabdomyosarcomas, as well as other sarcomas, carcinomas, and neuroectodermal tumors are generally negative. In normal tissues, the antigen is strongly expressed in thymocytes and a subset of pancreatic islet cells.
Immunogen
Tissue / cell preparation (purified E-rosette forming cells from human peripheral blood lymphocytes).
Key Feature
Clonality
Monoclonal
Isotype
IgM
Clone Number
HO36-1.1
Host Species
Mouse
Tested Applications
IHC-P
IHC-P: 1/25 - 1/75. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
4267 CD99 P14209 MIC2 MIC2X MIC2Y 313470
Target
CD99
Cellular Localization
Cell Membrane
Application
Application
Formalin fixed paraffin embedded human tonsil stained with CD99/MIC2 antibody .
Positive Control
Tonsil
Application Notes
IHC-P: 1/25 - 1/75. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.