| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal [3E1.1] to Cdc2The cell division control protein (cdc2) kinase is a catalytic subunit of a protein kinase complex, called the M phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In the fission yeast Schizosaccharomyces pombe, the gene Cdc2 is responsible for controlling the transition from G1 phase to the S phase and from the G2 phase to the M phase of the cell cycle. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex that is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. |
| Immunogen | C terminal fragment, starting from methionine 85 of Xenopus Cdc2 . |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3E1.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at an assay dependent dilution. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Xenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | cdc2-prov CDC2 380246 MGC53829 cdc2prov cdc2 prov |
|---|---|
| Target | CDC2 |
| Tissue Specificity | 3E1.1 reacts strongly with immunoblotted antigen but does not immunoprecipitate native p34cdc2. |
| Cellular Localization | Nuclear |
| Application Notes | WB: Use at an assay dependent dilution. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS |
