Application
Western Blot of Human Cells is tested byApplication
Formalin-fixed, paraffin-embedded human tonsil stained with CDC2 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphocytes in the germinal center.| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to CDC2p34cdc2(a catalytic subunit of Maturation Promoting Factor) plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. |
| Immunogen | C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | A17.1.1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PInhibitionIPWB |
| For IF: Use at an assay dependent dilution. For IHC-P: Use at a concentration of 2-4μg/ml for 30min at RT. For Inhib (Inhibits activation of p34cdc2 kinase by cyclins): Use at an assay dependent dilution. For IP (Native and denatured): Use at a concentartion of 2μg/mg lysate. For WB: Use at a concentration of 2-4μg/ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatGuinea PigWoodchuck |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CDC2 CDC28A DKFZp686L20222 MGC111195 P34CDC2 |
|---|---|
| Target | CDK1 |
| Cellular Localization | Cytoplasmic |
Application
Western Blot of Human Cells is tested by
Application
Formalin-fixed, paraffin-embedded human tonsil stained with CDC2 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphocytes in the germinal center.| Positive Control | HeLa, MAD109, PC12 or NIH-3T3 Cells, Tonsil, or Lymph Node |
|---|---|
| Application Notes | For IF: Use at an assay dependent dilution. For IHC-P: Use at a concentration of 2-4μg/ml for 30min at RT. For Inhib (Inhibits activation of p34cdc2 kinase by cyclins): Use at an assay dependent dilution. For IP (Native and denatured): Use at a concentartion of 2μg/mg lysate. For WB: Use at a concentration of 2-4μg/ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 200ug/ml of antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. |
