Application
Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1) .| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal [POH - 1] to Cdc2Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8). |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | POH-1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkeyMinkBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 P06493 DKFZp686L20222 116940 CDK1 983 MGC111195 |
|---|---|
| Target | CDC2 |
| Tissue Specificity | Reacts with Cdc2 |
Application
Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1) .| Positive Control | HeLa. |
|---|---|
| Application Notes | ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
