Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
CDC23 antibody [N2C2], Internal detects CDC23 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. CDC23 antibody [N2C2], Internal dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CDC23(APC8) antibody at 1:200 dilution.| Product Name | CDC23 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC23 (cell division cycle 23 homolog (S. cerevisiae))The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC23. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, BovineMouse (99%), Rat (99%), Zebrafish (84%), Xenopus laevis (93%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANAPC8 antibody APC8 antibody CUT23 antibody CDC23 antibody anaphase promoting complex subunit 8 antibody cyclosome subunit 8 antibody cell division cycle protein 23 homolog antibody anaphase-promoting complex subunit 8 antibody cell division cycle |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
CDC23 antibody [N2C2], Internal detects CDC23 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. CDC23 antibody [N2C2], Internal dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CDC23(APC8) antibody at 1:200 dilution.| Positive Control | H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
