Application
Sample (30 ug of whole cell lysate) A: H1299 B: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
CDC25A antibody detects CDC25A protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. CDC25A antibody diluted at 1:500.Application
CDC25A antibody detects CDC25A protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. CDC25A antibody diluted at 1:500.| Product Name | CDC25A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC25A (cell division cycle 25 homolog A (S. pombe))CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, BovineDog (89%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC25A2 antibody CDC25A antibody cell division cycle 25A antibody dual specificity phosphatase CDC25A antibody CDC25A2-CAG isoform antibody M-phase inducer phosphatase 1 antibody cell division cycle 25 homolog A (S. pombe) antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
CDC25A antibody detects CDC25A protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. CDC25A antibody diluted at 1:500.
Application
CDC25A antibody detects CDC25A protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. CDC25A antibody diluted at 1:500.| Positive Control | H1299 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
