| Product Name | CDC25A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 120] to Cdc25ACyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2. |
| Immunogen | Purified recombinant CDC25A. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-120 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| For IF: Use at an assay dependent diltion. For IHC: Use at 1:100 - 1:200. For IP (NativeUse Protein G): Use at a dilution of 1:400 protein lysate. For WB: Use at a dilution of 1:100 - 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | 993 116947 P30304 CDC25A2 CDC25A |
|---|---|
| Target | CDC25A |
| Cellular Localization | Nuclear membrane |
| Positive Control | LS174T cells, Tonsil |
|---|---|
| Application Notes | For IF: Use at an assay dependent diltion. For IHC: Use at 1:100 - 1:200. For IP (NativeUse Protein G): Use at a dilution of 1:400 protein lysate. For WB: Use at a dilution of 1:100 - 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and |
