Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000| Product Name | CDC25B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC25B (cell division cycle 25 homolog B (S. pombe))CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CDC25B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (93%), Rat (92%), Pig (93%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC25B antibody M-phase inducer phosphatase 2 antibody dual specificity phosphatase Cdc25B antibody cell division cycle 25B antibody cell division cycle 25 homolog B (S. pombe) antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (11) |
| Cellular Localization | Centrosome |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
