Application
WB analysis of (1) K562, (2) U937 and (3) THP-1 lysates (10 μg per lane) using Cdc25B antibody [EPR3459(2)] at a dilution of 1:1,000.| Product Name | Cdc25B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR3459(2)] to Cdc25BCDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide corresponding to residues in human Cdc25B |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR3459(2) |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC25B cell division cycle 25B |
|---|---|
| Target | Cdc25B |
Application
WB analysis of (1) K562, (2) U937 and (3) THP-1 lysates (10 μg per lane) using Cdc25B antibody [EPR3459(2)] at a dilution of 1:1,000.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | pH: 7.4, Preservative: 0.01% Sodium azide. Constituents: 50% Glycerol, 0.05% BSA |
