Application
K562 (Human chronic myelogenous leukemia cells) extract was separated on SDS-PAGE and probed with Mono AB to CDC25C CL:DCS-193 . The antibody was developed with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate and an NBT/BCIP substrate. Antibody dilution 1/1,000.| Product Name | CDC25C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 193] to Cdc25CThe activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1-S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DCS-193 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 65 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P30307 995 CDC25C 157680 CDC25 |
|---|---|
| Target | CDC25C |
Application
K562 (Human chronic myelogenous leukemia cells) extract was separated on SDS-PAGE and probed with Mono AB to CDC25C CL:DCS-193 . The antibody was developed with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate and an NBT/BCIP substrate. Antibody dilution 1/1,000.| Positive Control | K562 cell extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 65 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide |
