Application
Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDC34 antibody at 1:100 dilution.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using CDC34 antibody at 1:200 dilution.| Product Name | CDC34 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [14B5] to CDC34Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis. |
| Immunogen | Full-length human Cdc34 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 14B5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 116948 997 CDC34 UBE2R1 P49427 E2CDC34 E2-CDC34 E2 CDC34 |
|---|---|
| Target | CDC34 |
| Light Chain Type | kappa |
Application
Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDC34 antibody at 1:100 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using CDC34 antibody at 1:200 dilution.| Positive Control | 293 cells and recombinant fusion protein |
|---|---|
| Application Notes | Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added. |
