Application
CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.Application
Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CDC42EP3 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CDC42EP3 (N-term)CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC1). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 2~32 amino acids from the N-term region of human CDC42EP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSE55related Cdc42binding protein CDC42EP3 BORG2 CEP3 CDC42EP3 Binder of Rho GTPases 2 MSE55-related Cdc42-binding protein Binder of Rho GTPases2 Cdc42 effector protein 3 Cdc42 effector protein3 |
|---|---|
| Target | CDC42EP3 |
| Molecular Weight(MW) | 27.678 kDa (note) |
Application
CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
