Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000Application
CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC45L protein stained by CDC45L antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Product Name | CDC45L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC45L (CDC45 cell division cycle 45-like (S. cerevisiae))The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC45L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (92%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC45L antibody CDC45L2 antibody PORC-PI-1 antibody CDC45 antibody CDC45-related protein antibody cell division cycle 45-like 2 antibody human CDC45 antibody cell division control protein 45 homolog antibody CDC45 cell division cycle 45 homolog ant |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (3) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000
Application
CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC45L protein stained by CDC45L antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Positive Control | A549 , HeLa , *AGS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
