Application
Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody| Product Name | CDC47 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to CDC47 / MCM7This antibody reacts with a 80 kD protein known as hCDC47. hCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts. This antibody cross reacts with mouse, rat, dog and Xenopus. |
| Immunogen | BALB/C mice were injected with recombinant human CDC47 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 47DC141 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation, Western Blotting: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P33993 PNAS146 MCM2 CDC47 P1.1-MCM3 4176 P1CDC47 CDABP0042 600592 P1.1MCM3 MCM7 P85MCM PNAS-146 PNAS 146 P1.1 MCM3 |
|---|---|
| Target | CDC47 |
| Cellular Localization | Nuclear |
Application
Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation, Western Blotting: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
