Application
Whole extract of A431 cells was separated on SDS-PAGE, blotted with 0.4 μg/mL Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.| Product Name | CDC7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 341] to Cdc7 KinaseDNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The involvement of the latter complex in DNA replication was shown by different assays such as injection of antibodies against Cdc7 kinase that caused inhibition of DNA replication. Furthermore, mutant mice lacking mouse Cdc7 kinase are early embryonic lethal at E3.5-6.5.1-3 Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The protein shares about 45% identity with yeast Cdc7 kinase in the kinase domain. Cdc7 kinase is inert as a kinase unless it is activated by the ASK protein (the human homologue of yeast Dbf4). The expression of ASK is tightly regulated with cell cycle. ASK protein level is very low at the M-G1 phase and increases at late G1, staying high during S phase and through G2 phase. As a consequence, the activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). The phosphorylation of proteins of the MCM complex by Cdc7 kinase is important for complex reorganization and duplex unwinding activity in the replication fork. Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DCS-341 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | CDC7 HsCDC7 MGC126237 603311 8317 CDC7L1 huCDC7 MGC126238 Hsk1 O00311 MGC117361 |
|---|---|
| Target | CDC7 |
| Cellular Localization | Nuclear |
Application
Whole extract of A431 cells was separated on SDS-PAGE, blotted with 0.4 μg/mL Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
