Application
IHC ImageImmunohistochemistry on human tonsil using| Product Name | CDC7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [SPM171] to Cdc7 KinaseDNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SPM171 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| IHC-P: 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | CDC7 HsCDC7 MGC126237 603311 8317 CDC7L1 huCDC7 MGC126238 Hsk1 O00311 MGC117361 |
|---|---|
| Target | CDC7 |
| Light Chain Type | kappa |
Application
IHC ImageImmunohistochemistry on human tonsil using| Positive Control | Hela cells Tonsil |
|---|---|
| Application Notes | IHC-P: 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC |
| Storage Buffer | Preservative: Sodium Azide. Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
