Application
Western blot analysis of extracts from 293 cells, using CDCA2 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | CDCA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CDCA2Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDCA2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cell division cycle associated2 MGC129907 CDCA2 MGC129906 cell division cycle associated 2 FLJ25804 |
|---|---|
| Target | CDCA2 |
| Molecular Weight(MW) | 112 kDa (note) |
| Tissue Specificity | CDCA2 Antibody detects endogenous levels of total CDCA2 protein. |
| Cellular Localization | Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As |
Application
Western blot analysis of extracts from 293 cells, using CDCA2 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
