Application
Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.Application
CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CDCA3 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CDCA3 (N-term)F-box-like protein which is required for entry into mitosis. Acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase. |
| Immunogen | KLH conjugated synthetic peptide between 48~77 amino acids from the N-terminal region of human CDCA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | C8 CDCA3 TOME1 Trigger of mitotic entry protein1 Cell division cycleassociated protein3 Cell division cycleassociated protein 3 Generich cluster protein C8 Cell division cycle-associated protein 3 Gene-rich cluster protein C8 TOME-1 GRCC8 Trigge |
|---|---|
| Target | CDCA3 |
| Molecular Weight(MW) | 28.998 kDa (note) |
Application
Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.
Application
CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
