Application
Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDCA3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDCA3; C8; GRCC8; TOME1; Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C8 GRCC8 TOME1 |
|---|---|
| Alternative Names | CDCA3 C8 GRCC8 TOME1 Cell division cycle-associated protein 3 Gene-rich cluster protein C8 Trigger of mitotic entry protein 1 |
| Molecular Weight(MW) | 28998 Da |
| Function | F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity) |
| Tissue Specificity | This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the N-terminal region of human CDCA3. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). |
| Entrez Gene | 83461 |
|---|
Application
Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
