Application
FZR1 antibody detects FZR1 protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. FZR1 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE diluted at 1:1000Application
FZR1 antibody detects FZR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using CDH1 antibody at 1:500 dilution.Application
FZR1 antibody detects FZR1 protein at nucleus in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. FZR1 antibody diluted at 1:500.| Product Name | CDH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDH1 (fizzy/cell division cycle 20 related 1 (Drosophila))Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDH1. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (95%), Xenopus laevis (90%), Pig (97%), Chicken (94%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC20C antibody CDH1 antibody FZR antibody FZR2 antibody HCDH antibody HCDH1 antibody KIAA1242 antibody FZR1 antibody CDC20-like 1b antibody fizzy-related protein homolog antibody CDC20-like protein 1 antibody cdh1/Hct1 homolog antibody fizzy/c |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Isoform 2: Nucleus , Isoform 3: Cytoplasm |
Application
FZR1 antibody detects FZR1 protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. FZR1 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE diluted at 1:1000
Application
FZR1 antibody detects FZR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using CDH1 antibody at 1:500 dilution.
Application
FZR1 antibody detects FZR1 protein at nucleus in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. FZR1 antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
