Application
Formalin fixed paraffin embedded human spleen stained with Cdk2 antibody .| Product Name | CDK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2B6] to Cdk2Cdk2 complexes with cyclins E, D1 and D3. Cyclin E-cdk2 kinase is active in the G1 and S-phases of the cell cycle and is important (as does Cyclin A-cdk2) for the progression from G1 to S-phase. The levels of Cyclin A-cdk2 are maximal at the G1/S transition and both cdk2 and Cyclin A are found associated with DNA in the initiation complex during replication. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2B6 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PWB |
| IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | P24941 p33(CDK2) 116953 1017 CDK2 |
|---|---|
| Target | CDK2 |
| Tissue Specificity | This antibody reacts with a 33 kD protein known as cdk2. |
| Cellular Localization | Nuclear, cytoplasmic |
Application
Formalin fixed paraffin embedded human spleen stained with Cdk2 antibody .| Positive Control | Tonsil |
|---|---|
| Application Notes | IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4-8 °C. |
| Storage Buffer | PBS, pH7.2 containing 0.05% sodium azide |
