Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK5 monoclonal antibody.Application
HEK293T cells transfected with either CDK5 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK5 antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5.Application
Anti-CDK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CDK5Application
Immunofluorescent staining of HT29 cells using anti-CDK5 mouse monoclonal antibody .| Product Name | CDK5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H6] to CDK5Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase. |
| Immunogen | Protein expressed in 293T cell transfected with human CDK5 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PSSALRE |
|---|---|
| Target | CDK5 |
| Tissue Specificity | is specific for human CDK5. |
| Cellular Localization | In axonal growth cone with extension to the peripheral lamellipodia. |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK5 monoclonal antibody.
Application
HEK293T cells transfected with either CDK5 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK5 antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5.
Application
Anti-CDK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CDK5
Application
Immunofluorescent staining of HT29 cells using anti-CDK5 mouse monoclonal antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
