Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5 antibody at 1:200 dilution.Application
CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. CDK5 antibody [C2C3], C-term dilution: 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5 antibody at 1:100 dilution.Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500.| Product Name | CDK5 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDK5 (cyclin-dependent kinase 5)Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Sheep, Rhesus Monkey, BovinePig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PSSALRE antibody CDK5 antibody tau protein kinase II catalytic subunit antibody cyclin-dependent kinase 5 antibody protein kinase CDK5 splicing antibody TPKII catalytic subunit antibody cell division protein kinase 5 antibody serine/threonine-protei |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell projection , lamellipodium , growth cone |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5 antibody at 1:200 dilution.
Application
CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. CDK5 antibody [C2C3], C-term dilution: 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5 antibody at 1:100 dilution.
Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500.| Positive Control | A431 , H1299 , HeLa , Jurkat , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
