Application
Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) AntibodyApplication
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Product Name | CDK6 (Phospho Tyr24) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDK6 (Phospho Tyr24)Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y(p)-G-K) derived from Human CDK6. |
| Modification | p-Tyr24 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC59692 PLSTIRE CDK6 cyclin-dependent kinase 6 serine/threonine-protein kinase PLSTIRE cell division protein kinase 6 |
|---|---|
| Target | CDK6 (Phospho Tyr24) |
| Molecular Weight(MW) | 36 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) Antibody
Application
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
