Application
CDK8 antibody [C3], C-term detects CDK8 protein at cytoplasm and nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. CDK8 antibody [C3], C-term diluted at 1:500.Application
CDK8 antibody detects CDK8 protein by western blot analysis. Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK8 antibody diluted at a dilution of 1:3000.| Product Name | CDK8 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CDK8 (cyclin-dependent kinase 8)The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Human, Rat, Zebrafish, Xenopus laevis, Rhesus MonkeyHuman (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | K35 antibody MGC126074 antibody MGC126075 antibody CDK8 antibody CDK8 protein kinase antibody mediator of RNA polymerase II transcription subunit CDK8 antibody mediator complex subunit CDK8 antibody cyclin-dependent kinase 8 antibody protein kinase |
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| Target | CDK8 |
| Molecular Weight(MW) | 53 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
CDK8 antibody [C3], C-term detects CDK8 protein at cytoplasm and nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. CDK8 antibody [C3], C-term diluted at 1:500.
Application
CDK8 antibody detects CDK8 protein by western blot analysis. Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK8 antibody diluted at a dilution of 1:3000.| Positive Control | A549 , H1299 , HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
