Application
WB analysis of extracts from various cell lines using CDK9 antibody.Application
ICC/IF analysis of U2OS cells using CDK9 antibody (red) and DAPI (blue).| Product Name | CDK9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CDK9The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human CDK9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PITALRE C-2k cyclin-dependent kinase 9 CDC2L4 TAK CTK1 |
|---|---|
| Target | CDK9 |
| Molecular Weight(MW) | 43 kDa (note) |
Application
WB analysis of extracts from various cell lines using CDK9 antibody.
Application
ICC/IF analysis of U2OS cells using CDK9 antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
