| Product Name | CDT2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDT2 (denticleless homolog (Drosophila))Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CDT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Predicted Reactivity | Human, Mouse, Rhesus MonkeyHuman (100%), Mouse (88%), Rhesus Monkey (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDT2 antibody DCAF2 antibody L2DTL antibody RAMP antibody DTL antibody DDB1 and CUL4 associated factor 2 antibody denticleless protein homolog antibody RA-regulated nuclear matrix-associated protein antibody DDB1- and CUL4-associated factor 2 antib |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) |
| Cellular Localization | Nucleus membrane , Cytoplasm |
| Positive Control | Target recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
