Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDX2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2 antibody at 1:250 dilution.| Product Name | CDX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDX2 (caudal type homeobox 2)The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (93%), Rat (97%), Pig (98%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDX-3 antibody CDX3 antibody CDX2 antibody caudal type homeo box transcription factor 2 antibody caudal-type homeobox protein 2 antibody homeobox protein CDX-2 antibody caudal type homeobox transcription factor 2 antibody caudal type homeobox 2 anti |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDX2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2 antibody at 1:250 dilution.| Positive Control | H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
