WB
Western blot analysis of extracts from COLO cells, using CEP135 Antibody. The lane on the right is treated with the synthesized peptide.ICC/IF
Immunofluorescence analysis of A549 cells using CEP135 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.| Product Name | CEP135 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CEP135 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CEP135. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Centrosomal protein of135 kDa Centrosomal protein 4 Centrosomal protein4 CEP135 Centrosomal protein of 135 kDa |
|---|---|
| Target | CEP135 |
| Molecular Weight(MW) | 133 kDa (note) |
| Tissue Specificity | CEP135 Antibody detects endogenous levels of total CEP135 protein. |
WB
Western blot analysis of extracts from COLO cells, using CEP135 Antibody. The lane on the right is treated with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of A549 cells using CEP135 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
