Application
CEP152 antibody detects CEP152 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 5 % SDS-PAGE CEP152 antibody dilution: 1:1000Application
Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 μg of CEP152 antibody . Western blot analysis was performed using CEP152 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
CEP152 antibody detects CEP152 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: CEP152 protein stained by CEP152 antibody diluted at 1:2000. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody diluted at 1:5000. Blue: Hoechst 33342 staining.| Product Name | CEP152 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CEP152 (centrosomal protein 152kDa)This gene encodes a protein that is thought to be involved with centrosome function. Mutations in this gene have been associated with primary microcephaly (MCPH4). Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CEP152. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21594 antibody KIAA0912 antibody MCPH4 antibody CEP152 antibody centrosomal protein of 152 kDa antibody centrosomal protein 152kDa antibody |
|---|---|
| Molecular Weight(MW) | 189 kDa (note) (2) |
| Cellular Localization | Centrosome |
Application
CEP152 antibody detects CEP152 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 5 % SDS-PAGE CEP152 antibody dilution: 1:1000
Application
Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 μg of CEP152 antibody . Western blot analysis was performed using CEP152 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
CEP152 antibody detects CEP152 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: CEP152 protein stained by CEP152 antibody diluted at 1:2000. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody diluted at 1:5000. Blue: Hoechst 33342 staining.| Positive Control | 293T , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
