Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CERK antibody at 1:500 dilution.| Product Name | CERK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CERK (ceramide kinase)CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CERK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (88%), Rat (90%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434E0211 antibody FLJ21430 antibody FLJ23239 antibody KIAA1646 antibody LK4 antibody MGC131878 antibody dA59H18.2 antibody dA59H18.3 antibody hCERK antibody CERK antibody ceramide kinase antibody lipid kinase 4 antibody acylsphingosine kin |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CERK antibody at 1:500 dilution.| Positive Control | H1299 , HeLa , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
