Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CES2 antibody at 1:500 dilution.Application
CES2 antibody detects CES2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CES2 protein stained by CES2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CES2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CES2 (carboxylesterase 2)Carboxylesterase 2 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CES2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CE-2 antibody CES2A1 antibody PCE-2 antibody iCE antibody CES2 antibody intestinal carboxylesterase liver carboxylesterase-2 antibody hCE-2 antibody carboxylesterase 2 (intestine liver) antibody methylumbelliferyl-acetate deacetylase 2 antibody |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CES2 antibody at 1:500 dilution.
Application
CES2 antibody detects CES2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CES2 protein stained by CES2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
