Application
staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)Application
c-fos antibody at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal| Product Name | c-Fos antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c-FosThe products of the proto-oncogenes c-Jun (c-Jun was previously known as the Fos-binding protein p39) and c-Fos are known to form a complex in the nucleus (2, 6). AP-1 (activating protein-1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF (activating transcription factor) subunits that bind to a common DNA site, the AP-1-binding site (1). There is evidence that AP-1 proteins, mostly those that belong to the Jun group, control cell life and death through their ability to regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16 (3). The Fos and Jun proto-oncogenes expression is induced transiently by a great variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons (5). Studies have established a unique role for Fos in determining the differentiation and activity of progenitors of the osteoclast lineage (4). |
| Immunogen | The immunogen corresponding to a region within amino acids 1 and 50 of human c-Fos. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 2353 FOS 164810 c-fos cFos P01100 c Fos |
|---|---|
| Target | c-Fos |
| Tissue Specificity | Reacts with c-Fos |
Application
staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
Application
c-fos antibody at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal| Positive Control | A-431 and HeLa lysate |
|---|---|
| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IHC:1:10~1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing 0.1% sodium azide and 0.2%gelatin |
