Application
Western blot analysis of CGBP at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system and by exposing to the film for 15 minutes.| Product Name | CGBP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CGBPHuman CpG binding protein (hCGBP) contains three cysteine-rich domains, two of which exhibit homology to the plant homeodomain finger domain and the third cysteine-rich domain conforms to the CXXC motif identified in DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1. A fragment of hCGBP that contains the CXXC domain binds to an oligonucleotide probe containing a single CpG site, and this complex is disrupted by distinct oligonucleotide competitors that also contain a CpG motif(s). CBGP plays an important part in embryonic viability and peri-implantation development. Native hCGBP is detected as an 88 kDa protein by Western analysis and is ubiquitously expressed. CGBP trans-activates promoters that contain CpG motifs but not promoters in which the CpG is ablated suggesting that hCGBP is a transcriptional activator that recognizes unmethylated CpG dinucleotides. |
| Immunogen | Synthetic peptides, CREKDPKLEIRYRHKKSR and IQHDPLTTDLRSSADR corresponding to amino acids 73-90 and 641-656 of Human CGBP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 88 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 609150 30827 Q9P0U4 HsT2645 5830420C16Rik hCGBP CGBP 2410002I16Rik PHF18 PCCX1 CXXC1 |
|---|---|
| Target | CGBP |
| Cellular Localization | Nuclear |
Application
Western blot analysis of CGBP at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system and by exposing to the film for 15 minutes.| Positive Control | Human spleen cell lysate. |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 88 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
